MISSION

CHROMAVISION aims to develop a pioneering chromosome imaging and manipulation platform that will fuel the next decades of structural chromosome research. Chromosomal abnormalities are characteristic of many disorders such as cancer, impaired fertility due to maternal aging, and neurological disorders such as fragile X syndrome. If humanity is to fully understand the wide range of diseases that are associated to errors in cell division, we must be able to further ‘zoom in’ on healthy and diseased chromosomes in all their complexity. The CHROMAVISION platform will allow molecular biologists to automatically isolate individual chromosomes from small tissue or cell samples and have these delivered to a super-resolution microscope. Chromosome isolation and delivery is achieved by an opto-fluidic chip that is able to trap, visualise and lyse individual cells and separate metaphase chromosomes from cell lysate. Single chromo- somes can be “hand-selected” and brought into focus of the Super-Resolution Correlative Tweezers Fluorescence Microscope (CTFM-SR3D) that is developed in CHROMAVISION. This instrument will for the first time enable 3D, super-resolution, real-time metaphase chromosome observation and manipulation studies under near physiological conditions. The technique will push the boundaries of what is currently possible in microfluidics and super-resolution microscopy and combine these into a single powerful approach for chromosome studies. Furthermore, the platform will be applied in CHROMAVISION to address key challenges in clinical and fundamental chromosome research, potentially resulting in breakthrough discoveries. Better imaging and understanding of the chromosomal mechanisms will contribute to our knowledge of the etiology of human diseases and aid drug discovery. The platform will also have large clinical value, allowing identification and monitoring of e.g. cancer heterogeneity.

CHROMAVISION flow-chart

flowchart

EU FUNDING

“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 665233.”

CONSORTIUM

The CHROMAVISION platform will allow molecular biologists to automatically isolate individual chromosomes from small tissue or cell samples and have these delivered to a super-resolution imaging system; the Super-Resolution Correlative Tweezers Fluorescence Microscope (CTFM-SR3D). This instrument will for the first time enable 3D, super-resolution observation of metaphase chromosomes in real time, and permit manipulation of these chromosomes under near-physiological conditions. This technique will push the boundaries of what is currently possible in microfluidics and super-resolution microscopy, and will combine these into a single powerful approach for chromosome studies. To achieve the goals of the Chromavision platform, interdisciplinary research is needed. Therefore five specific partners have decided to collaborate in this project. Together they are the perfect match. Below we introduce the five partners. They will shortly explain the core business of the institute/company and go into their research related to the project.

Prof. Dr. Ian Hickson
Center for Chromosome Stability

UNIVERSITY OF COPENHAGEN
Department of Cellular and Molecular Medicine

Core business The Hickson/Liu laboratory is based in the Center for Chromosome Stability (CCS) at the University of Copenhagen. The CCS was established in 2015 through a grant of 8.5 Mio Euro from the Danish National Research Foundation. The CCS’s...

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Prof. Dr. Anders Kristensen
Optofluidics

DENMARK TECHNICAL UNIVERSITY

Core business The research includes photonic crystal sensor surfaces for refractometric imaging, opto-thermal actuation and laser manipulation, nanoplasmonics for integrated delivery of light at the nanoscale, and plasmonic color metasurfaces for decoration and laser nano-lithography. This research is supported by...

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PROF. DR. G.J.L. (GIJS) WUITE
PHYSICS OF LIVING SYSTEMS

VRIJE UNIVERSITEIT AMSTERDAM
Department of Physics and Astronomy (coordinator of the project)

Core businessMy group is part of the Physics of Living Systems Section in the faculty of Sciences at the Vrije Universiteit Amsterdam. The research in the group focuses on exploring DNA-proteins interactions and biophysical/biomechanical properties of viral capsids & cells....

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OLIVIER HEYNING
Capture Molecular Interactions

LUMICKS

Core business LUMICKS is a spin-off company from the Vrije Universiteit Amsterdam, bringing to market the revolutionary C-Trap Correlative Tweezers (CTFM) and Acoustic Force Spectroscope (AFS). The instruments enable live visualization of DNA-protein interactions at single molecule level in real...

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Prof. Dr. Charles Swanton
OPTOFLUIDICS

University College London

Core business The Swanton laboratory focuses on mechanisms generating cancer genetic diversity and its consequences on clinical outcome. Our group and others, through next generation sequencing studies, have demonstrated that the principles of Darwinian evolution apply to the growth and...

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contact

Chromavision

Vrije Universiteit Amsterdam
Faculty of Science – Physics and Astronomy
Attn. Sandrijn van Roemburg
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
Phone: +31 (0)20 59 87891
Email: s.j.h.van.roemburg@vu.nl